Performance Evaluation of Kawsar DNA Banking Card (DBC) for Preservation of DNA from Multiple Biological Matrices: Application in PCR-Based Assays

Danial Taghipour; Hasan Moayeri; Khashayar Behzadi; Simin Zarei; Mozhdeh Hajiveisi; Maryam Shahi Ardakani

doi:10.63053/ijhes.215

Authors

Danial Taghipour Department of Veterinary medicine, Sa.C., Islamic Azad University, Sanandaj, Iran
Hasan Moayeri Department of General Surgery, Kurdistan University of Medical Sciences, Sanandaj, Iran
Khashayar Behzadi Department of Veterinary medicine, Sa.C., Islamic Azad University, Sanandaj, Iran
Simin Zarei Cancer and immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
Mozhdeh Hajiveisi Department of Veterinary medicine, Sa.C., Islamic Azad University, Sanandaj, Iran
Maryam Shahi Ardakani Faculty of Humanities, Islamic Azad University, Sanandaj, Iran

DOI:

https://doi.org/10.63053/ijhes.215

Keywords:

DNA, Specimen shipping, Polymerase Chain Reaction, Inhibitors, Quality Control

Abstract

DNA preservation cards provide a practical alternative to traditional cold storage methods for molecular applications. This study assessed the performance of the locally produced Kawsar DNA Banking Card (DBC) for preserving DNA from whole blood, saliva, tissue, cell lysate, and tissue homogenate at room temperature for one month. DNA quality was evaluated by spectrophotometry and PCR amplification of the human GAPDH gene.

Washed punches yielded measurable DNA concentrations (53.59–188.81 ng/µL), though with low purity ratios. Direct PCR from unwashed punches showed minimal amplification. By using the washing step, clear GAPDH bands (235 bp) were obtained from saliva, tissue, and cell lysate samples, while tissue homogenate mainly produced off-target products. All DBC-derived signals were weaker than the positive control.

The Kawsar DBC effectively preserves amplifiable DNA from various biological matrices when a washing procedure is applied. It offers a cost-effective, room-temperature solution suitable for biobanking and PCR-based diagnostics, particularly in resource-limited settings

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