The Investigation of drug release systems for application Probiotic Delivery


  • Ghazaleh Akhavan
  • Rana Imani


probiotics,, release system, , hydrogels, microcapsules


Probiotics are live microorganisms that have a positive effect on our health and bring many benefits when consumed in sufficient doses. Maintaining the viability of probiotic bacteria during oral administration can be challenging due to the harsh conditions they face, such as the acidic conditions of the stomach. However, delivery systems for probiotics are very different and important in terms of effectiveness for patient health. These release systems can be categorized into conventional formulations, pharmaceuticals, and non-conventional products, mainly food-based commercial products. In this review, we focus on polymeric carriers and methods applied to encapsulate probiotics in them. Microcapsule technology has been proposed as a successful strategy with key factors including the ability of microcapsules to transport viable functional bacteria in sufficient numbers, protect against harsh physiological conditions, and survive formulation processes to improve their efficacy after oral administration. Also, biodegradable polymers or hydrogels as carriers of probiotics can protect bacteria from the acidic environment of the stomach and increase their survival during storage and consumption. Overall, by combining advances in carrier materials and microcapsules, there is promising potential to improve probiotic delivery and increase health benefits for patients.



Augustin, M.A.; Hemar, Y. (2009) Nano- and micro-structured assemblies for encapsulation of food ingredients. Chem. Soc. Rev., 38, 902–912.

Aditya, N.P.; Espinosa, Y.G.; Norton, I.T. (2017) Encapsulation systems for the delivery of hydrophilic nutraceuticals: Food application. Biotechnology. Adv., 35, 450–457.

C. Hill, F. Guarner, G. Reid, G.R. Gibson, D.J. Merenstein, B. Pot, L. Morelli, R.B. Canani, H.J. Flint, S. Salminen, (2014) Expert consensus document: the international scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic, Nat. Rev. Gastroenterol. Hepatol.11 506–514.

G.Deshpande, G. Athalye_Jape, S.Patole, (2018), Para-Probiotics for preterm neonates, the next frontier, Nutrients,10,871-875

S. Asgari, A. Pourjavadi, T.R. Licht, et al., (2020) Polymeric carriers for enhanced delivery of probiotics, Adv. Drug Delivery. Rev., 4-12.

K. J. Chua, W. C. Kwok, N. Aggarwal, T. Sun and M. W. Chang, ( 2017) Designer probiotics for the prevention and treatment of human diseases, Curr. Opin. Chem. Biol, 40, 8–16.

Bosch M, Nart J, Audivert S, Bonachera MA, Alemany AS, Fuentes MC, et al. (2012) Isolation and characterization of probiotic strains for improving oral health. Arch Oral Biol. 57(5):539–49.

Reiff C, Kelly D. (2010) Inflammatory bowel disease, gut bacteria, and probiotic therapy. Int J Food Microbiol. 300 (1):25–33.

Solanki, H.K.; Pawar, D.D.; Shah, D.A.; Prajapati, V.D.; Jani, G.K.; Mulla, A.M.; Thakar, P.M. (2013)Development of Microencapsulation Delivery System for Long-Term Preservation of Probiotics as Biotherapeutics Agent. Biomed. Res. Int. 2013, 620719.

M.R.I. Shishir, L. Xie, C. Sun, X. Zheng, W. Chen, (2018) Advances in micro and nano-encapsulation of bioactive compounds using biopolymer and lipid-based transporters, Trends Food Sci. Technol., 7834-60.

S. Chen, Q. Zhao, L.R. Ferguson, Q. Shu, I. Weir, S. Garg,(2012) Development of a novel probiotic delivery system based on microencapsulation with protectants, Appl. Microbiol. Biotechnol., 93,1447-1457.

A. Homayouni, A. Azizi, M. Ehsani, M. Yarmand, S. Razavi, (2008) Effect of microencapsulation and resistant starch on the probiotic survival and sensory properties of synbiotic ice cream, Food chem., 111,50-55.

V. Manojlović, V.A. Nedović, K. Kailasapathy, N.J. Zuidam, (2010) Encapsulation of probiotics for use in food products, Encapsulation technologies for active food ingredients and food processing, Springer, pp. 269-302.

V.G. Yadav, (2019) A probiotic for treating cancer, Sci. Transl. Med., 11 eaay3576.

R. Nagpal, A. Kumar, M. Kumar, P.V. Behare, S. Jain, H. Yadav, (2012) Probiotics, their health benefits and applications for developing healthier foods: a review, FEMS Microbiol. Lett., 334, 1-15.

Y. Byun, Y. T. Kim, K. G. H. Desai, and H. J. Park, (2010) Microencapsulation techniques for food flavor, Chem. Biol. Volatiles, 307–332.

Seyedehhamideh Razavi, Sajjad Janfaza, Nishat Tasnim, Deanna L. Gibsonbc, and Mina Hoorfar. (2021), Nanomaterial-based encapsulation for controlled gastrointestinal delivery of viable probiotic bacteria, Nanoscale Adv., 3, 2699.

H. Zhang, C. Yang, W. Zhou, Q. Luan, W. Li, Q. Deng, X. Dong, H. Tang and F. Huang, (2018), A pH-responsive gel macrosphere based on sodium alginate and cellulose nanofiber for potential intestinal delivery of probiotics, ACS Sustainable Chem. Eng., 6, 13924–13931.

T. Huq, C. Fraschini, A. Khan, B. Riedl, J. Bouchard and M. Lacroix, (2017), Alginate based nanocomposite for microencapsulation of probiotic: Effect of cellulose nanocrystal (CNC) and lecithin, Carbohydr. Polym., 168, 61–69.

W. Krasaekoopt, B. Bhandari and H. C. (2006), Deeth, Survival of probiotics encapsulated in chitosan-coated alginate beads in yogurt from UHT-and conventionally treated milk during storage, LWT–Food Sci. Technol., 39, 177–183.

A. Mawad, Y. A. Helmy, A.-G. Shalkami, D. Kathayat, G. Rajashekara and E. coli, (2018), Nissle microencapsulation in alginate-chitosan nanoparticles and its effect on Campylobacter jejuni in vitro, Appl. Microbiol. Biotechnol., 102, 10675–10690.

L. A. Soares and E. Crema, (2020), Study of a delayed-release system for hard and soft capsules coated with eudragit® s100 acrylic polymers, Acta Sci., Health Sci., 42, e48422.

M. Yao, B. Li, H. Ye, W. Huang, Q. Luo, H. Xiao, D. J. McClements and L. Li, (2018), Enhanced viability of probiotics (Pediococcus pentosaceus Li05) by encapsulation in microgels doped with inorganic nanoparticles, Food Hydrocolloids, 83, 246–252.

M. A. OdeniyiA, O. A. OmotesoB, A. O. AdepojuB and K. T. JaiyeobaE, (2018), Starch nanoparticles in drug delivery: A review, Polim. Med., 48, 41–45.

S. Thangrongthong, N. Puttarat, B. Ladda, T. Itthisoponkul, W. Pinket, K. Kasemwong and M. Taweechotipatr, (2020), Microencapsulation of probiotic Lactobacillus brevis ST-69 producing GABA using alginate supplemented with nanocrystalline starch, Food Sci. Biotechnol., 29, 1475–148226.

K. S. Prakash, R. Chavan and V. Mishra, (2016), Microencapsulation of Probiotics and its Applications, Frontier Discoveries and Innovations in Interdisciplinary Microbiology, Springer, pp. 33–44.

K. Feng, M.-Y. Zhai, Y. Zhang, R. J. Linhardt, M.-H. Zong, L. Li, and H. Wu,(2018), Improved viability and thermal stability of the probiotics encapsulated in a novel electrospun fiber mat, J. Agric. Food Chem., 66, 10890–10897.

Govender, M.; Choonara, Y.E.; Kumar, P.; du Toit, L.C.; van Vuuren, S.; Pillay, V. (2014), A Review of the Advancements in Probiotic Delivery: Conventional vs. Non-conventional Formulations for Intestinal Flora Supplementation. AAPS PharmSciTech 15, 29–43.

Bajpai, S.K.; Kirar, N. (2016), Swelling and drug release behavior of calcium alginate/poly (sodium acrylate) hydrogel beads. Des. Monomers Polym.19, 89–98.

Sohail, A.; Turner, M.S.; Coombes, A.; Bostrom, T.; Bhandari, B. (2011), Survivability of probiotics encapsulated in alginate gel microbeads using a novel impinging aerosols method. Int. J. Food Microbiol.145, 162–168.

Ding, W.K.; Shah, N.P. Shah. (2007), Heat Tolerance of Free and Microencapsulated Probiotic Bacteria. J. Food Sci.72, 446–450.

A.C. Anselmo, K.J. McHugh, J. Webster, R. Langer, A. Jaklenec, (2016) Layer‐by‐layer encapsulation of probiotics for delivery to the microbiome, Adv. Mater., 28, 9486-9490.

J. Shen, Z.-X. Zuo, A.-P. Mao, Effect of probiotics on inducing remission and maintaining therapy in ulcerative colitis, Crohn's disease, and pouchitis: a meta-analysis of randomized controlled trials, Inflamm. Bowel Dis., 20 (2014) 21-35.

P. Panigrahi, S. Parida, N.C. Nanda, R. Satpathy, L. Pradhan, D.S. Chandel, L. Baccaglini, A. Mohapatra, S.S. Mohapatra, P.R. Misra, A randomized synbiotic trial to prevent sepsis among infants in rural India, Nature, 548 (2017) 407-412.



How to Cite

Akhavan , G., & Imani, R. (2023). The Investigation of drug release systems for application Probiotic Delivery. International Journal of New Findings in Health and Educational Sciences (IJHES), 1(2), 88–96. Retrieved from