Investigating the effect of astaxanthin on the survival rate of MCF-7 breast cancer cells by MTT method (cytotoxicity test)

Fatemeh Teimourpour; Ghazal Rahmani; Bahar Fathalahi; Hadis  Shafiei

doi:10.63053/ijhes.63

Authors

Fatemeh Teimourpour Department of Biology, Islamic Azad University, Science and Research Unit, Tehran, Iran
Ghazal Rahmani Department of Biology, Nedaya Rehaneh Educational Institute, Tehran, Iran
Bahar Fathalahi Department of Biology, Iran Nuclear Industry Institute, Tehran, Iran
Hadis Shafiei Department of Biology, Nedaya Rehaneh Educational Institute, Tehran, Iran

DOI:

https://doi.org/10.63053/ijhes.63

Abstract

Cancer is known as a very dangerous and incurable disease, with symptoms such as pain and disability. In recent years, changes in the way people live have increased the prevalence of breast cancer in different societies. This terrible disease has increased research in this field. Astaxanthin is one of the most famous natural carotenoids, which, due to its strong antioxidant activity, can prevent cardiovascular diseases, strengthen the body's immune system, and prevent cell ageing. In this study, the effect of astaxanthin on MCF-7 cancer cells was investigated. After culturing the cells in 96-well plates, they were treated with different concentrations of astaxanthin and then evaluated using the MTT method. The results showed that astaxanthin can inhibit cell growth and induce apoptosis, and this effect is enhanced by increasing the concentration of astaxanthin, so that at a concentration of 100 μM, the rate of apoptosis increased to 35%. Therefore, it can be concluded that astaxanthin may be used as a control treatment for cancer.

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