Investigating the effect of astaxanthin on the survival rate of MCF-7 breast cancer cells by MTT method (cytotoxicity test)
DOI:
https://doi.org/10.63053/ijhes.63Keywords:
astaxanthin, MCF-7 cancer cell, MTTAbstract
Cancer is known as a very dangerous and incurable disease, with symptoms such as pain and disability. In recent years, changes in the way people live have increased the prevalence of breast cancer in different societies. This terrible disease has increased research in this field. Astaxanthin is one of the most famous natural carotenoids, which, due to its strong antioxidant activity, can prevent cardiovascular diseases, strengthen the body's immune system, and prevent cell ageing. In this study, the effect of astaxanthin on MCF-7 cancer cells was investigated. After culturing the cells in 96-well plates, they were treated with different concentrations of astaxanthin and then evaluated using the MTT method. The results showed that astaxanthin can inhibit cell growth and induce apoptosis, and this effect is enhanced by increasing the concentration of astaxanthin, so that at a concentration of 100 μM, the rate of apoptosis increased to 35%. Therefore, it can be concluded that astaxanthin may be used as a control treatment for cancer.
References
Cancer is known as a very dangerous and incurable disease, with symptoms such as pain and disability. In recent years, changes in the way people live have increased the prevalence of breast cancer in different societies. This terrible disease has increased research in this field. Astaxanthin is one of the most famous natural carotenoids, which, due to its strong antioxidant activity, can prevent cardiovascular diseases, strengthen the body's immune system, and prevent cell ageing. In this study, the effect of astaxanthin on MCF-7 cancer cells was investigated. After culturing the cells in 96-well plates, they were treated with different concentrations of astaxanthin and then evaluated using the MTT method. The results showed that astaxanthin can inhibit cell growth and induce apoptosis, and this effect is enhanced by increasing the concentration of astaxanthin, so that at a concentration of 100 μM, the rate of apoptosis increased to 35%. Therefore, it can be concluded that astaxanthin may be used as a control treatment for cancer.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 International Journal of New Findings in Health and Educational Sciences (IJHES)
This work is licensed under a Creative Commons Attribution 4.0 International License.
The journal is licensed under a Attribution 4.0 International (CC BY 4.0).
You are free to:
- Share — copy and redistribute the material in any medium or format for any purpose, even commercially.
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
- The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution - You must give appropriate credit , provide a link to the license, and indicate if changes were made . You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions - You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
