Evaluation of antimicrobial effects of copper–zinc oxide nanocomposites biosynthesized by pome pomegranate peel on some pathogenic bacteria

Rahil  Alaghehband; Leila Tavanaei*

doi:10.63053/ijhes.134

Authors

Rahil Alaghehband Behbahan faculty of medical sciences,Behbahan,Iran
Leila Tavanaei* Behbahan faculty of medical sciences,Behbahan,Iran

DOI:

https://doi.org/10.63053/ijhes.134

Keywords:

pathogenic bacteria, biosynthesis, antibacterial activity, copper and zinc oxide nanocomposite

Abstract

Ever since humans started using antibiotics to treat bacterial diseases, resistance to treatment has been observed in bacteria. Studies have shown that by using metals we can take advantage of their antibacterial effects. The purpose of this research is to investigate the antibacterial activities of copper-zinc nanocomposite synthesized by green method (from pomegranate peel extract) on some pathogenic bacteria. Therfore, after green synthesis of copper-zinc oxide nanocomposite, the characteristics of nanocomposite were evaluated using UV-Vis spectrophotometry, X-ray diffraction analysis (XRD), FT-IR analysis, spectrum analysis (EDX) and scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Antibacterial activity of zinc-copper oxide nanocomposite against some pathogenic agents was investigated by well diffusion and blank disk methods in agar medium. The results of Well diffusion method were better compared to blank disc. It was demonstrated that the maximum inhibitory concentration on Staphylococcus aureus bacteria at concentrations of 100, 250, and 500 µg/ml showed; diameters of the non-growth halo of 17, 16 and 21 mm respectively. In contrast, the minimum inhibitory concentration on Enterococcus faecalis at concentrations of 100, 250, and 500 µg/ml, showed the diameters of the non-growth halo of 10, 12, and 14 mm respectively. The microbicidal properties of nanoparticles were investigated by two methods: the minimum growth inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). In the MIC method, the treatment concentration of 0.78 PPM stopped the growth of Escherichia coli and the treatment concentration of 1.56 PPM stopped the growth of other bacteria. MBC for Escherichia coli was 1.56 and for Pseudomonas aeruginosa, Shigella dysentery and Staphylococcus aureus was 3.12 PPM and had no effect on Enterococcus faecalis. These results showed that copper-zinc oxide nanocomposite synthesized from pomegranate peel inhibited the growth of the above-mentioned bacteria in all concentrations.

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