The Effect of a Single About of Resistance Exercise with Two Intensities and Different Recovery on Pro-Inflammatory Factor TNF-Α, HSP-70, And Anti-Inflammatory Factor BDNF In Female Bodybuilders
DOI:
https://doi.org/10.63053/ijhes.88Keywords:
Resistance training; Brain-Derived Neurotrophic Factor; HSP70 Heat-Shock Proteins; Tumor Necrosis Factor-alphaAbstract
Background and purpose: resistance exercises with high loads of HLRT can cause physiological changes, including in the immune system. The purpose and credibility of qualitative research investigated the effect of a resistance activity session with two different intensities and recovery on the expression of TNF-α, HSP-70, and BDNF genes in female bodybuilders.
Methods: In this Quasi-experimental study, twelve healthy female bodybuilders (age 30±5) volunteered to participate in the research. The samples performed one weekly strength training protocol session designed at two intensities of one maximum repetition in two different recovery times of one and two minutes. 36 hours after the end of the training, sampling was done after ten hours of fasting, and the samples were centrifuged and stored at minus 20℃. At the end of the four stages, the levels of TNF-α, HSP-70, and BDNF were checked in the medical laboratory. A consent form was obtained from all the participants. SPSS version 27 software was used for data analysis at a significance level of α ≤ 0.05.
Findings: The results showed that HLRT resistance training compared to moderate intensity resistance training, by increasing the training intensity to 85% of a maximum repetition and recovery time of one and two minutes, significantly reduced TNF-α level (with p<0.001and a significant increase in the serum levels of BDNF and HSP-70 (with p<0.0001).
Conclusion: It seems that increasing the intensity of HLRT resistance training has no negative effect on the immune system, and probably with the decrease of the pro-inflammatory factor TNF-α and HSP-70, the anti-inflammatory factor BDNF also increased reduces the incidence of inflammation in female bodybuilders.
References
Yuan, J., et al., Application and progress of blood flow restriction training in improving muscle mass and strength in the elderly. Frontiers in Physiology, 2023. 14: p. 1155314. DOI: https://doi.org/10.3389/fphys.2023.1155314
Anjum, M.M., et al., Overcoming barriers in cystic fibrosis therapy through inhalational lipid nanoparticles: Challenges and advances. Journal of Drug Delivery Science and Technology, 2023: p. 105068. DOI: https://doi.org/10.1016/j.jddst.2023.105068
Sarellis, S.D., The Effects of High-Load Versus Low-Load Resistance Training on Isokinetic Knee Extensor and Flexor Peak Power, Vastus Intermedius, and Vastus Lateralis Muscle Thickness in Untrained Overweight and Obese Adults. 2020, Arizona State University.
Kambic, T., et al., Protocol: Effects of high-load and low-load resistance training in patients with coronary artery disease: rationale and design of a randomised controlled clinical trial. BMJ Open, 2021. 11(7). DOI: https://doi.org/10.1136/bmjopen-2021-051325
Calle, M.C. and M.L. Fernandez, Effects of resistance training on the inflammatory response. Nutrition research and practice, 2010. 4(4): p. 259-269. DOI: https://doi.org/10.4162/nrp.2010.4.4.259
Kitsuda, Y., et al., Impact of high-load resistance training on bone mineral density in osteoporosis and osteopenia: a meta-analysis. Journal of bone and mineral metabolism, 2021. 39: p. 787-803. DOI: https://doi.org/10.1007/s00774-021-01218-1
Sarkar, S., et al., Effect of high intensity interval training on antioxidant status, inflammatory response and muscle damage indices in endurance team male players. Apunts Sports Medicine, 2021. 56(210): p. 100352. DOI: https://doi.org/10.1016/j.apunsm.2021.100352
Liu, Y., et al., Short-term resistance exercise inhibits neuroinflammation and attenuates neuropathological changes in 3xTg Alzheimer’s disease mice. Journal of neuroinflammation, 2020. 17: p. 1-16. DOI: https://doi.org/10.1186/s12974-019-1653-7
Borde, R., T. Hortobágyi, and U. Granacher, Dose–response relationships of resistance training in healthy old adults: a systematic review and meta-analysis. Sports medicine, 2015. 45: p. 1693-1720. DOI: https://doi.org/10.1007/s40279-015-0385-9
Metsios, G.S., R.H. Moe, and G.D. Kitas, Exercise and inflammation. Best practice & research Clinical rheumatology, 2020. 34(2): p. 101504. DOI: https://doi.org/10.1016/j.berh.2020.101504
Azizbeigi, K., et al., Effect of moderate and high resistance training intensity on indices of inflammatory and oxidative stress. Research in sports medicine, 2015. 23(1): p. 73-87. DOI: https://doi.org/10.1080/15438627.2014.975807
Wang, S., et al., Effect of exercise training on body composition and inflammatory cytokine levels in overweight and obese individuals: a systematic review and network meta-analysis. Frontiers in immunology, 2022. 13: p. 921085. DOI: https://doi.org/10.3389/fimmu.2022.921085
Cerqueira, É., et al., Inflammatory effects of high and moderate intensity exercise—a systematic review. Frontiers in physiology, 2020. 10: p. 1550. DOI: https://doi.org/10.3389/fphys.2019.01550
Beutler, B. and A. Cerami, The biology of cachectin/TNF--a primary mediator of the host response. Annual review of immunology, 1989. 7(1): p. 625-655. DOI: https://doi.org/10.1146/annurev.iy.07.040189.003205
Vassalli, P., The pathophysiology of tumor necrosis factors. Annual review of immunology, 1992. 10(1): p. 411-452. DOI: https://doi.org/10.1146/annurev.immunol.10.1.411
Carneiro, M.A., et al., Effects of resistance training at different loads on inflammatory biomarkers, muscle mass, muscular strength, and physical performance in postmenopausal women. The Journal of Strength & Conditioning Research, 2022. 36(6): p. 1582-1590. DOI: https://doi.org/10.1519/JSC.0000000000003768
Kim, S.-D. and Y.-R. Yeun, Effects of Resistance Training on C-Reactive Protein and Inflammatory Cytokines in Elderly Adults: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. International Journal of Environmental Research and Public Health, 2022. 19(6): p. 3434. DOI: https://doi.org/10.3390/ijerph19063434
Benavente, C., et al., Hormonal and inflammatory responses to hypertrophy-oriented resistance training at acute moderate altitude. International Journal of Environmental Research and Public Health, 2021. 18(8): p. 4233. DOI: https://doi.org/10.3390/ijerph18084233
Akbulut, T., et al., The role of different exercises in irisin, heat shock protein 70 and some biochemical parameters. Journal of Medical Biochemistry, 2022. 41(2): p. 149. DOI: https://doi.org/10.5937/jomb0-31551
Lu, J., et al., High-intensity interval training alleviates exhaustive exercise-induced HSP70-assisted selective autophagy in skeletal muscle. The Journal of Physiological Sciences, 2023. 73(1): p. 32. DOI: https://doi.org/10.1186/s12576-023-00884-2
Krüger, K., T. Reichel, and C. Zeilinger, Role of heat shock proteins 70/90 in exercise physiology and exercise immunology and their diagnostic potential in sports. Journal of Applied Physiology, 2019. 126(4): p. 916-927. DOI: https://doi.org/10.1152/japplphysiol.01052.2018
Dimauro, I., et al., Systemic response of antioxidants, heat shock proteins, and inflammatory biomarkers to short-lasting exercise training in healthy male subjects. Oxidative medicine and cellular longevity, 2021. 2021. DOI: https://doi.org/10.1155/2021/1938492
Mogharnasi, M., et al., The Effects of resistance and endurance training on levels of nesfatin-1, HSP70, insulin resistance and body composition in women with type 2 diabetes mellitus. Science & Sports, 2019. 34(1): p. e15-e23. DOI: https://doi.org/10.1016/j.scispo.2018.04.010
Quiles, J.M., et al., Impact of resistance training program configuration on the circulating brain-derived neurotrophic factor response. Applied Physiology, Nutrition, and Metabolism, 2020. 45(6): p. 667-674. DOI: https://doi.org/10.1139/apnm-2019-0419
Arazi, H., et al., Acute effects of strength and endurance exercise on serum BDNF and IGF-1 levels in older men. BMC geriatrics, 2021. 21: p. 1-8. DOI: https://doi.org/10.1186/s12877-020-01937-6
Silveira-Rodrigues, J.G., et al., Acute bouts of aerobic and resistance exercise similarly alter inhibitory control and response time while inversely modifying plasma BDNF concentrations in middle-aged and older adults with type 2 diabetes. Experimental Brain Research, 2023. 241(4): p. 1173-1183. DOI: https://doi.org/10.1007/s00221-023-06588-8
Du, X., et al., The effects of low-intensity resistance training with or without blood flow restriction on serum BDNF, VEGF and perception in patients with post-stroke depression. Neuroendocrinology Letters, 2021. 42(4): p. 229-235.
Benini, R., et al., Influence of sex on cytokines, heat shock protein and oxidative stress markers in response to an acute total body resistance exercise protocol. Journal of Exercise Science & Fitness, 2015. 13(1): p. 1-7. DOI: https://doi.org/10.1016/j.jesf.2014.10.002
Moran, J., et al., A meta-analysis of resistance training in female youth: its effect on muscular strength, and shortcomings in the literature. Sports Medicine, 2018. 48: p. 1661-1671. DOI: https://doi.org/10.1007/s40279-018-0914-4
Carneiro, M.A., et al., Effect of whole-body resistance training at different load intensities on circulating inflammatory biomarkers, body fat, muscular strength, and physical performance in postmenopausal women. Applied Physiology, Nutrition, and Metabolism, 2021. 46(8): p. 925-933. DOI: https://doi.org/10.1139/apnm-2020-0746
Sabouri, M., et al., Inflammatory, antioxidant and glycemic status to different mode of high-intensity training in type 2 diabetes mellitus. Molecular Biology Reports, 2021. 48: p. 5291-5304. DOI: https://doi.org/10.1007/s11033-021-06539-y
Nogueira, M.E., et al., High-protein diet associated with resistance training reduces cardiac TNF-α levels and up-regulates MMP-2 activity in rats. Archives of Physiology and Biochemistry, 2022. 128(6): p. 1630-1636. DOI: https://doi.org/10.1080/13813455.2020.1787456
Soltani, N., et al., Resistance exercise training augments the immunomodulatory adaptations to aerobic high-intensity interval training: HIIT prescription, TLR4 pathway, and negative regulatory proteins. European Journal of Sport Science, 2023(just-accepted): p. 1-24.
Gonzalez-Gil, A.M. and L. Elizondo-Montemayor, The role of exercise in the interplay between myokines, hepatokines, osteokines, adipokines, and modulation of inflammation for energy substrate redistribution and fat mass loss: a review. Nutrients, 2020. 12(6): p. 1899. DOI: https://doi.org/10.3390/nu12061899
Ihalainen, J.K., A. Hackney, and R. Taipale, Changes in inflammation markers after a 10-week high-intensity combined strength and endurance training block in women: The effect of hormonal contraceptive use. Journal of science and medicine in sport, 2019. 22(9): p. 1044-1048. DOI: https://doi.org/10.1016/j.jsams.2019.04.002
Mardanpour Shahrekordi, Z., E. Banitalebi, and M. Faramarzi, The effect of resistance training on levels of interlukine-6 and high-sensitivity C-reactive protein in older-aged women. Elderly Health Journal, 2017. 3(1): p. 35-41.
Kamiya, M., et al., Low-intensity resistance training to improve knee extension strength in community-dwelling older adults: systematic review and meta-analysis of randomized controlled studies. Experimental Gerontology, 2023. 172: p. 112041. DOI: https://doi.org/10.1016/j.exger.2022.112041
Kehrer, P., K. Kelly, and N. Heffernan, Does Immediate Feedback While Doing Homework Improve Learning? Grantee Submission, 2013.
Zarei, M., A. Pejhan, and S.M. Beheshti-Nasr, Comparison of the Acute Response of Some Inflammatory Markers to Circular Resistance Training in Fasting in the Morning and Evening in Obese Men. Journal of Isfahan Medical School, 2020. 37(560): p. 1440-1447.
Tavvafian, N., et al., Effects of glycyrrhizic acid supplementation during nonlinear resistance training on inflammatory markers and muscular damage indices in overweight young men. Obesity Medicine, 2020. 17: p. 100178. DOI: https://doi.org/10.1016/j.obmed.2019.100178
Morano, K.A., New tricks for an old dog: the evolving world of Hsp70. Annals of the New York Academy of Sciences, 2007. 1113(1): p. 1-14. DOI: https://doi.org/10.1196/annals.1391.018
Nameni, F., The Effect of a Single Bout Endurance Training on HSP70. World Applied Sciences Journal, 2012. 19(2): p. 211-4.
Murlasits, Z., The role of heat shock proteins in skeletal muscle adaptation to resistance training in young and old rats. 2005: West Virginia University.
Nameni, F., The Effect of One Bout Endurance And Acute Exercise on HSP 70 in Female. World Applied Sciences Journal, 2012. 18(5): p. 727-730.
Church, D.D., et al., Comparison of high-intensity vs. high-volume resistance training on the BDNF response to exercise. Journal of Applied Physiology, 2016. DOI: https://doi.org/10.1152/japplphysiol.00233.2016
Eidukaitė, S., N. Masiulis, and M. Kvedaras, Exploring the Preliminary Effects of Resistance Training on Total Brain-Derived Neurotrophic Factor (BDNF) Levels in Elderly Individuals: A Pilot Study. Baltic Journal of Sport and Health Sciences, 2023. 2(129): p. 4-10. DOI: https://doi.org/10.33607/bjshs.v2i129.1377
Setayesh, S. and G.R.M. Rahimi, The impact of resistance training on brain-derived neurotrophic factor and depression among older adults aged 60 years or older: A systematic review and meta-analysis of randomized controlled trials. Geriatric Nursing, 2023. 54: p. 23-31. DOI: https://doi.org/10.1016/j.gerinurse.2023.08.022
Silva, B.S.D.A., et al., Inflammatory and metabolic responses to different resistance training on chronic obstructive pulmonary disease: a randomized control trial. Frontiers in physiology, 2018. 9: p. 262. DOI: https://doi.org/10.3389/fphys.2018.00262
Nielsen, A.R. and B.K. Pedersen, The biological roles of exercise-induced cytokines: IL-6, IL-8, and IL-15. Applied physiology, nutrition, and metabolism, 2007. 32(5): p. 833-839. DOI: https://doi.org/10.1139/H07-054
Sardeli, A.V., et al., Effect of resistance training on inflammatory markers of older adults: A meta-analysis. Experimental gerontology, 2018. 111: p. 188-196. DOI: https://doi.org/10.1016/j.exger.2018.07.021
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